Enzyme purification using two-phase aqueous partitioning Leucine dehydrogenase is recovered from 150
1 answer below » Enzyme purification using two-phase aqueous partitioning Leucine dehydrogenase is recovered from 150 litres of Bacillus cereus homogenate using an aqueous two-phase polyethylene glycol-salt system. The homogenate initially contains 3.2 units of enzyme ml-1. A polyethylene glycol-salt mixture is added and two phases form. The enzyme partition coefficient is 3.5. (a) What volume ratio of upper and lower phases must be chosen to achieve 80% recovery of enzyme in a single extraction step? (b) If the volume of the lower phase is 100 litres, what is the concentration factor for 80% recovery? Jul 08 2022 02:25 PM
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